新乡医学院学报 , Journal of Xinxiang Medical College, 编辑部邮箱 , 2011年06期 【作者】 杜伟奇; 施秀芳; 张文博; 张振中;
【Author】 DU Wei-qi1,SHI Xiu-fang2,ZHANG Wen-bo3,ZHANG Zhen-zhong2(1.Drug and Equipment Section,Anyang Regional Hospital,Anyang 455000,Henan Province,China;2.School of Pharmaceutical Science,Zhengzhou University,Zhengzhou 450001,Henan Province,China;3.Department of Life Science and Technology,Xinxiang Medical College,Xinxiang 453003,Henan Province,China)
【机构】 安阳地区医院药械科; 郑州大学药学院; 新乡医学院生命科学技术系;
【摘要】 目的建立高效液相色谱法测定十味降糖泡腾颗粒中葛根素含量的方法。方法采用Hypersil C18色谱柱(250.0 mm×4.6 mm,5.0μm),甲醇-0.025 mol.L-1磷酸盐(pH 3.5)(14)为流动相,检测波长为250 nm,流速1.0 mL.min-1。结果理论塔板数按葛根素峰计算为7 600,供试品中葛根素能与其他杂质峰很好的分离,分离度R>1.5,峰形对称,拖尾因子为1。葛根素2.515~100.600 mg.L-1呈良好的线性关系(r=0.999 9),平均加样回收率和相对标准偏差分别为98.58%和2.40%。结论本法准确可靠,可用于测定十味降糖泡腾颗粒中葛根素的含量。 更多还原
【Abstract】 Objective To establish a quantitative analysis method of puerarin in Shiwei Jiangtang effervescent granules by high performance liquid chromatography(HPLC).Methods HPLC with Hypersil C18 column(250.0 mm×4.6 mm,5.0 μm) was used,methanol-0.025 mol·L-1 phosphate(pH 3.5)(14) as the mobile phase,at the detection wavelength of 250 nm and the flow rate of 1.0 mL·min-1.Results The number of theoretical plates of the column was 7 600,calculated with reference to the peak of puerarin.The puerarin of test... 更多还原 【关键词】 十味降糖泡腾颗粒; 葛根素; 高效液相色谱法; 含量测定; 【Key words】 Shiwei Jiangtang effervescent granules; puerarin; high performance liquid chromatography; determination; 【分类号】R286.0
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