临床肝胆病杂志 , Journal of Clinical Hepatology, 编辑部邮箱 , 2011年10期 【作者】 陆小蒟; 陈永平; 阳韬; 申春燕;
【Author】 LU Xiao-ju,CHEN Yong-ping,YANG Tao,et al.(Department of Infectious Diseases,THE First Affiliated Hospital of Wenzhou Medical College,Wenzhou,Zhejiang 325000,China)
【机构】 温州医学院附属第一医院感染科;
【摘要】 目的观察急性肝衰竭大鼠肝脏NF-κB的表达水平及黄芩苷对其表达的影响。方法雄性SD大鼠106只随机分为正常组、肝衰竭组和黄芩苷组。肝衰竭组和黄芩苷组采用腹腔注射D-氨基半乳糖制备大鼠急性肝衰竭模型;黄芩苷组于造模后每隔12 h以120 mg/kg剂量腹腔注射。造模后24 h、72 h、120 h和168 h处死大鼠。全自动生化仪检测血清ALT、AST和TBil水平;HE染色观察肝脏病理学变化;RT-PCR法检测肝组织NF-κB、TNFα、Caspase-3 mRNA表达;免疫组化法检测肝组织NF-κB蛋白表达。结果黄芩苷组大鼠168 h存活率明显高于肝衰竭组。黄芩苷组大鼠各时间点血清ALT、AST、TBil水平较肝衰竭组明显降低(F=173.584,158.329,74.902;P<0.01)。黄芩苷组NF-κB、TNFα、Caspase-3 mRNA表达趋势与肝衰竭组相同,72 h达高峰,但表达量较肝衰竭组明显减少,差异有统计学意义(F=603.801,42.174,27.222,P<0.001,P<0.001,P=0.001)。黄芩苷组NF-κB蛋白的表达也于72... 更多还原
【Abstract】 Objective To observe the expression of NF-κB in acute liver failure in rats and the effect of baicalin on the expression of NF-κB.Methods 106 male SD rats were randomly divided into normal group,model group and baicalin group.The model group and baicalin group produced the animal model of acute liver failure by intraperitoneal injection with D-galactosamine(D-GalN).Baicalin(120 mg/kg) was treated intraperitoneally(i.p.) at every 12 h after D-GalN was injected in rats.We chose 6 rats randomly at ... 更多还原 【关键词】 肝功能衰竭,急性; 黄芩苷; NF-κB; 疾病模型,动物; 【Key words】 liver failure,acute; Baicalin; NF-kappa B; disease models,animal; 【分类号】R285.5
中华医学之家:http://www.xinxi85.com
投稿信箱:zhyxzj85@163.com
联系电话:029--85327298 主编QQ:693891972 |